| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.197989 |
| Chromosome: | chromosome 12 |
| Location: | 6452163 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538050 | (1 of 7) PF07004 - Sperm-tail PG-rich repeat (SHIPPO-rpt) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTTGTGTATCTATTTTATATGTACGTG |
| Internal bar code: | GTTGCTCGCAACACACGCTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 99.5671 |
| LEAP-Seq n confirming: | 230 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTTGTGTGATGGGACAG |
| Suggested primer 2: | CAGCAAGACTGGTATCGCAA |