Insertion junction: LMJ.RY0402.197993_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.197993
Chromosome:chromosome 11
Location:793462
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467638 (1 of 2) PTHR27000:SF119 - LEUCINE-RICH REPEAT RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE BAM2-RELATED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAATGATCACACGCGGCATGTCGTGGGCG
Internal bar code:CTGGGCCAGCTTCCGCCGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:750
LEAP-Seq percent confirming:98.8636
LEAP-Seq n confirming:261
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGCACACAATGCGATTCAAC
Suggested primer 2:AGTACAGCACACCCCAGTCC