| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198006 |
| Chromosome: | chromosome 10 |
| Location: | 3302820 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443500 | PCD3 | Polyketide cyclase/dehydrase; (1 of 4) PF03364 - Polyketide cyclase / dehydrase and lipid transport (Polyketide_cyc) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCAATCCGTGTACCATACATTCCATGG |
| Internal bar code: | GCAACTAACCCTCATTGTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 98.9971 |
| LEAP-Seq n confirming: | 1382 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTAACCCTGTTTCGCCAT |
| Suggested primer 2: | CACTCGCTTCATCTTCGTGA |