| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198034 |
| Chromosome: | chromosome 2 |
| Location: | 4699158 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101750 | (1 of 1) PF04484 - Family of unknown function (DUF566) (DUF566) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAAAATGACGCCTCTTGTATGTCCGTAT |
| Internal bar code: | GAATAAATCCCGGCACAAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.513 |
| LEAP-Seq n confirming: | 1839 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGTTCTGGATGGCAAGGG |
| Suggested primer 2: | AGTGCGATGCCTGATAGCTT |