| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198071 |
| Chromosome: | chromosome 9 |
| Location: | 4022346 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393025 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGATGCCCAGCTATCTACGGGTCCTCTG |
| Internal bar code: | GCGATTTGTACGTTGTGGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 96.3855 |
| LEAP-Seq n confirming: | 1520 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTCACGTTCCAAGCCAC |
| Suggested primer 2: | CCCAACTAACGCCCAACTTA |