| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198094 |
| Chromosome: | chromosome 10 |
| Location: | 2427086 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435750 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCACGCAGATGCCCTCATGCATGATGCCG |
| Internal bar code: | AGCTTTACTACACGCTCATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 7 |
| LEAP-Seq percent confirming: | 97.3333 |
| LEAP-Seq n confirming: | 146 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACCGCATACGTGTGAAT |
| Suggested primer 2: | GCATGCTCATGCTGTTCACT |