| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198111 |
| Chromosome: | chromosome 12 |
| Location: | 3483061 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496150 | (1 of 1) PTHR20953//PTHR20953:SF4 - KINASE-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGCGCGGGGGCCAGGGGACTTGGCAGG |
| Internal bar code: | GGGGGCTGATTGAGAAGGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 99.2553 |
| LEAP-Seq n confirming: | 933 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCTCTGCCCACTCTTGC |
| Suggested primer 2: | CATCTCCTCTCTCCCTGCTG |