| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198120 |
| Chromosome: | chromosome 2 |
| Location: | 5378473 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107300 | DHDPS,DPS1 | (1 of 1) 4.3.3.7 - 4-hydroxy-tetrahydrodipicolinate synthase / Dihydrodipicolinate synthetase; Dihydrodipicolinate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGACGTCGCGCATCGCAGAGTGCCTTT |
| Internal bar code: | CCGCAATATGCTGGGTCGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 76.5402 |
| LEAP-Seq n confirming: | 2199 |
| LEAP-Seq n nonconfirming: | 674 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGAATTGCTGCCTTAGCC |
| Suggested primer 2: | CACGAAGTGTTGATTGGGTG |