| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198137 |
| Chromosome: | chromosome_2 |
| Location: | 5811605 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g110950 | FAP133,DIC5 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGAATGGGGCGTGACTCTTGCAGGTGGAG |
| Internal bar code: | TAATTGGGAACGAGGGGAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 98.1381 |
| LEAP-Seq n confirming: | 13335 |
| LEAP-Seq n nonconfirming: | 253 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCAGGTTGTATCACCGT |
| Suggested primer 2: | GCGTGGAAGAGAAAGAATCG |