Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.198147 |
Chromosome: | chromosome_16 |
Location: | 3814285 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g689600 | FAP73 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAGCGCCCCGACATCCCGAGGCCTTTCTTA |
Internal bar code: | TACGTAATACGGGGTACTGCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1095 |
LEAP-Seq percent confirming: | 99.3569 |
LEAP-Seq n confirming: | 2472 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGACCACAAGGATGAGGA |
Suggested primer 2: | GGAAGGCTAGATGCACGAAG |