| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198156 |
| Chromosome: | chromosome 17 |
| Location: | 3168929 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721850 | SSA16 | cilia-sensing, structure and/or assembly; (1 of 1) K08292 - elongation factor 2 kinase (EEF2K) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGAGGAGGTGGTGGCGCTGAAGGGGCT |
| Internal bar code: | GGGTCCTGGTGGCTGTCGATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 99.8908 |
| LEAP-Seq n confirming: | 915 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACCCACAGAAATGTCC |
| Suggested primer 2: | CATGTACACCCATTCCCACA |