| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198199 |
| Chromosome: | chromosome 13 |
| Location: | 1546079 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572950 | E2F2,E2FR1 | Transcription factor, E2F and DP-related; (1 of 2) PTHR12548//PTHR12548:SF9 - TRANSCRIPTION FACTOR DP // TRANSCRIPTION FACTOR DP | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGTGCACCGCCACCCAGACCTTCTGCC |
| Internal bar code: | AAGGCGTTTTTAGTTTGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1053 |
| LEAP-Seq percent confirming: | 99.3516 |
| LEAP-Seq n confirming: | 5363 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATGCAACCGTCTTATTT |
| Suggested primer 2: | AGCTGTCGCTGAAATCTCGT |