| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.198209 |
| Chromosome: | chromosome 9 |
| Location: | 6928325 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGATGCAGGGCCCATTAACACGGGGCTT |
| Internal bar code: | CTCACGGACACCAAGAATCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 46.112 |
| LEAP-Seq n confirming: | 9583 |
| LEAP-Seq n nonconfirming: | 11199 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTGTTTCTGTGGTTGGG |
| Suggested primer 2: | TGAGCGTGCCAATTAGTCAG |