Insertion junction: LMJ.RY0402.198214_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.198214
Chromosome:chromosome 4
Location:2492168
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g221900 (1 of 9) IPR000210//IPR011042//IPR011333 - BTB/POZ domain // Six-bladed beta-propeller, TolB-like // POZ domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGACACGGACCGCGAAGTGCTGTACGGCGC
Internal bar code:TAGATATCGCTCATACACCCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:185
LEAP-Seq percent confirming:80.2794
LEAP-Seq n confirming:2011
LEAP-Seq n nonconfirming:494
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTTAAACGCAACACACAC
Suggested primer 2:AAGAAGTCGCTGTGGGAAGA