| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198216 |
| Chromosome: | chromosome 16 |
| Location: | 4573621 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688050 | (1 of 1) PTHR32091//PTHR32091:SF0 - FAMILY NOT NAMED // EUKARYOTIC INITIATION FACTOR 4B-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGTTTGTTTGACTTCGGTGACCTGGTG |
| Internal bar code: | AAAGCTTGGCTCGCGTACATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 99.9493 |
| LEAP-Seq n confirming: | 3944 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATTTGGTAGGGTCAGAT |
| Suggested primer 2: | TGATCCCCGTGTGTCAGTTA |