| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198236 |
| Chromosome: | chromosome_7 |
| Location: | 4754762 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g345400 | FAP70 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGCAATTGTGCAGGGCAGGAGGGGCCGG |
| Internal bar code: | TTCATCGCGCCTTAGCATAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 881 |
| LEAP-Seq percent confirming: | 97.8648 |
| LEAP-Seq n confirming: | 275 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACGAAGGAAAGCACAGG |
| Suggested primer 2: | GGCGTACACACAGGGTTTCT |