| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198244 |
| Chromosome: | chromosome 16 |
| Location: | 1867743 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655600 | (1 of 4) PF16016 - Domain of unknown function (DUF4782) (DUF4782) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCCACCTTGCCTCCTGATGTACCGTGT |
| Internal bar code: | TCCTGCAAGCGAGCCCCCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 99.851 |
| LEAP-Seq n confirming: | 670 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACGTACTGATCTCGGGG |
| Suggested primer 2: | CCAGTCCATGCCAACTACCT |