| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198245 |
| Chromosome: | chromosome 13 |
| Location: | 1918230 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576100 | (1 of 30) PTHR24349//PTHR24349:SF79 - SERINE/THREONINE-PROTEIN KINASE // NITROGEN NETWORK KINASE 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGAGTTGCACTGTGCTGCATGCTGCTG |
| Internal bar code: | GCCAGTGCTAGCCCTGTCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 991 |
| LEAP-Seq percent confirming: | 99.8636 |
| LEAP-Seq n confirming: | 2928 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTCGGGCTGCATAGTGTG |
| Suggested primer 2: | AAGGGTGGTCTAGGTTGCCT |