| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198247 |
| Chromosome: | chromosome 3 |
| Location: | 2528849 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160050 | FAP184 | (1 of 1) PTHR15654:SF1 - COILED-COIL DOMAIN-CONTAINING PROTEIN 96; Flagellar Associated Protein 184 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGGTGCTGGACGGCAACGCAGGGTCA |
| Internal bar code: | CGGGCGCATCCGGTGGGTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1120 |
| LEAP-Seq percent confirming: | 99.3792 |
| LEAP-Seq n confirming: | 10566 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGCGCGTTTATTGTATG |
| Suggested primer 2: | CACCTCATTGACTTCGAGCA |