Insertion junction: LMJ.RY0402.198250_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.198250
Chromosome:chromosome 3
Location:2671266
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g161100 (1 of 7) IPR011989//IPR016024 - Armadillo-like helical // Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAGCCCGACGTGCCGTACTGCACCGCCT
Internal bar code:CTCCGGCAATGTGACATTACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:195
LEAP-Seq percent confirming:99.0654
LEAP-Seq n confirming:106
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGGAGTTGGAGCAGGAG
Suggested primer 2:CGTACCGTGTCGGTCTAGGT