Insertion junction: LMJ.RY0402.198257_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.198257
Chromosome:chromosome 16
Location:6291044
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g674600 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTGAACTCCCGAGGTATTGCATACAGTA
Internal bar code:GGGAAGCTGGGTTGGCGGGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:594
LEAP-Seq percent confirming:97.2928
LEAP-Seq n confirming:14699
LEAP-Seq n nonconfirming:409
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGACTCAGATGACTGCTG
Suggested primer 2:GTGCTGAGCAGTGAAACGTC