| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198283 |
| Chromosome: | chromosome 12 |
| Location: | 4037144 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517300 | (1 of 1) PF07738 - Sad1 / UNC-like C-terminal (Sad1_UNC) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACCTGCTGTTGCACGCAGTACAGCAGG |
| Internal bar code: | GTAGGAGACGGCACCCTAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 98.5375 |
| LEAP-Seq n confirming: | 74789 |
| LEAP-Seq n nonconfirming: | 1110 |
| LEAP-Seq n unique pos: | 136 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTCTGCTGTCCATGCTG |
| Suggested primer 2: | TGCACCGTGGACAGATACAT |