Insertion junction: LMJ.RY0402.198336_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.198336
Chromosome:chromosome 7
Location:4106576
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g342950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGCAAATGCGACAGTACGGTACGGTAA
Internal bar code:TGTAGTACGGAATGGGCCTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAACTCGTCGAAGAACTCGG
Suggested primer 2:ACCCTTTCGTGTGTCAGTCC