| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198336 |
| Chromosome: | chromosome 10 |
| Location: | 5352362 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458100 | YEE1 | Membrane protein; (1 of 3) K07112 - uncharacterized protein (K07112) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGCATACACCGTAAACTGGGACGCCGT |
| Internal bar code: | TCACATGGGTTGTATTGGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 856 |
| LEAP-Seq percent confirming: | 99.7281 |
| LEAP-Seq n confirming: | 3668 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGTGAGAGTAGCGGTG |
| Suggested primer 2: | ATAACGCTGGACCCTCACAC |