Insertion junction: LMJ.RY0402.198345_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.198345
Chromosome:chromosome 2
Location:3907860
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g096300 CIA5,CCM1 Regulator of CO2-responsive genes 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCGAGCAGCAATAATTTAACCTATGAG
Internal bar code:AGTTGCTGAATTTCAGCGCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:28
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAAATTGCCACACAAACGTC
Suggested primer 2:GTGTGGTTGTTGTTTGACGG