Insertion junction: LMJ.RY0402.198345_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.198345
Chromosome:chromosome 12
Location:7662834
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g554400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTACTCAGTCGGTGCGTGCTCATCACGC
Internal bar code:ACCGAAGTCGTGGACACAACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:640
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCCGCTCCAATCTGCTAT
Suggested primer 2:CCCTTCATCAGCTTCTCCTG