| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198378 |
| Chromosome: | chromosome 1 |
| Location: | 2484288 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014050 | (1 of 1) PF16543 - DRG Family Regulatory Proteins, Tma46 (DFRP_C) | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGTATTATGTGCGATAATGGCTGAGGGT |
| Internal bar code: | CCATTTTTTTGCGACTCTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 261 |
| LEAP-Seq percent confirming: | 99.9336 |
| LEAP-Seq n confirming: | 3010 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGAACTACTAAAGGCCG |
| Suggested primer 2: | CATTTAGGCGTCTCAGCCTC |