Insertion junction: LMJ.RY0402.198386_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.198386
Chromosome:chromosome 13
Location:2741231
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g582600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAAGCACGGCGGACCCAGTCCCCTCACAC
Internal bar code:GGAAGGCGAAAGGTGAAGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:336
LEAP-Seq percent confirming:73.9648
LEAP-Seq n confirming:1679
LEAP-Seq n nonconfirming:591
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCAAACTCGTTGGAAGTG
Suggested primer 2:CTCCTCCTCCTCGTCCTCAT