| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198387 |
| Chromosome: | chromosome 12 |
| Location: | 77481 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483800 | CPY | Serine carboxypeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAGTGAGGGGCTAAAATTGTTGGGGGG |
| Internal bar code: | CGTCCGTGAAGTCCCGAGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 99.529 |
| LEAP-Seq n confirming: | 1902 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTGCTGGCTGTGTCATC |
| Suggested primer 2: | TCCACCAGGGACTGGTACTC |