Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.198389 |
Chromosome: | chromosome 2 |
Location: | 281318 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g075050 | PTA1 | (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCGCTCAGACGAGCGGCACGACGTACG |
Internal bar code: | ATATATTAAGTGTTTTCCAGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 534 |
LEAP-Seq percent confirming: | 87.4697 |
LEAP-Seq n confirming: | 2883 |
LEAP-Seq n nonconfirming: | 413 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCATGCCCTTAACACCTTC |
Suggested primer 2: | ACTGGGTTTCCAGGTCAGTG |