| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198403 |
| Chromosome: | chromosome 3 |
| Location: | 8448816 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199759 | GEX5,ORP3A | (1 of 1) PTHR10972:SF96 - OXYSTEROL-BINDING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACCAACTGTACCTACATTACAGCGTGT |
| Internal bar code: | GTTAGCGCCGGGCCGAAACGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 743 |
| LEAP-Seq percent confirming: | 99.6544 |
| LEAP-Seq n confirming: | 8650 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGTTGGGAGTGTGGAGT |
| Suggested primer 2: | ATACGTTAGGCCAAAATGCG |