| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198412 |
| Chromosome: | chromosome_10 |
| Location: | 5313634 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g457700 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCGTTCCTGAGTGCGAAGGTATCTGGCAA |
| Internal bar code: | AGATGACCTGCCTTGGCCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 984 |
| LEAP-Seq percent confirming: | 98.2036 |
| LEAP-Seq n confirming: | 984 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACTGAGGAACGAGCCTT |
| Suggested primer 2: | GCAGAGCACAATGGACAAGA |