| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198463 |
| Chromosome: | chromosome 17 |
| Location: | 2750199 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718400 | (1 of 1) IPR001810//IPR011050 - F-box domain // Pectin lyase fold/virulence factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTAAAGGCAAAGTGCGCTGCAGGCCGC |
| Internal bar code: | CGTTCGTCGACGATAATTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.7685 |
| LEAP-Seq n confirming: | 4741 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTTTACAGCTCAGCACA |
| Suggested primer 2: | TGTGAGGGTGAGATCCATCA |