| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198472 |
| Chromosome: | chromosome 7 |
| Location: | 1609371 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325000 | CYP18,CYP738A1 | Cytochrome P450, CYP120 superfamily; (1 of 4) 1.14.13.93 - (+)-abscisic acid 8'-hydroxylase / ABA 8'-hydroxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTATGAGTGGGAGGCGGTGAACCCAGCGG |
| Internal bar code: | GAGAGTTCTCAGCGCAAATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 876 |
| LEAP-Seq percent confirming: | 99.2471 |
| LEAP-Seq n confirming: | 18322 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATTTTTCGACACTGCGAG |
| Suggested primer 2: | CCAATACCAAGAGGGGGATT |