| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198486 |
| Chromosome: | chromosome 9 |
| Location: | 2792719 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389001 | (1 of 77) IPR001623 - DnaJ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGCAAGGGCTGCTACTCGCACCACCCC |
| Internal bar code: | CTCAAGCTAGATGAACATGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 75.4243 |
| LEAP-Seq n confirming: | 1200 |
| LEAP-Seq n nonconfirming: | 391 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGCCTGGTACAATACACT |
| Suggested primer 2: | GTCAAGGTGTATGTGTGCGG |