| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198490 |
| Chromosome: | chromosome 4 |
| Location: | 636328 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217946 | (1 of 1) PTHR22769:SF35 - NUDIX HYDROLASE FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGATGGCGTGTGCCAAAGCGACTTACA |
| Internal bar code: | CCGGGGCACAGCGTGCGATTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 938 |
| LEAP-Seq percent confirming: | 99.4467 |
| LEAP-Seq n confirming: | 2696 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAACTGTGGACCTTGGCAC |
| Suggested primer 2: | TTGCTACGCTTTGTTTGTGC |