| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.198512 |
| Chromosome: | chromosome_1 |
| Location: | 1822373 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009800 | FAP289 | Flagellar Associated Protein, coiled-coil | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCCAAAGTTATGGAACCACACGCATGACC |
| Internal bar code: | AGTACGGGTTCCGGGAGTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 536 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCATGTCGTACTACCCCG |
| Suggested primer 2: | ACTCGGGGTGATGAGTTTTG |