| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198529 |
| Chromosome: | chromosome 11 |
| Location: | 3199692 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGAACACCAACACCTTTCCTTGGGCCCC |
| Internal bar code: | GGGCCGAATAAGTGGTACGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 48.1712 |
| LEAP-Seq n confirming: | 2397 |
| LEAP-Seq n nonconfirming: | 2579 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACCAGCTCAATGAAGCG |
| Suggested primer 2: | TCCCTTTGCTCTTTTGTGCT |