| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198543 |
| Chromosome: | chromosome 6 |
| Location: | 1458085 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259401 | SMM16 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 2) PTHR10108//PTHR10108:SF813 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCCACCACGGTCTTGTGTCGCGGCGTG |
| Internal bar code: | AGCCCAAGGCTTGTATCGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1075 |
| LEAP-Seq percent confirming: | 99.7312 |
| LEAP-Seq n confirming: | 5937 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAAGTAGCTGTTTTGGGC |
| Suggested primer 2: | CCCACACCTACACCCATACC |