| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.198553 |
| Chromosome: | chromosome 3 |
| Location: | 6709462 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197600 | (1 of 1) PTHR12972//PTHR12972:SF0 - DOWNSTREAM NEIGHBOR OF SON // PROTEIN DOWNSTREAM NEIGHBOR OF SON | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGGCAAGTGCGGCGTGCGAGAGCGTGC |
| Internal bar code: | TATAGGGTTTGTCTCCATAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 99.2578 |
| LEAP-Seq n confirming: | 7890 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAGTGAGGTGGAGGTTG |
| Suggested primer 2: | CAGGACAAGACAGGGTTGGT |