| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198567 |
| Chromosome: | chromosome 12 |
| Location: | 6001044 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534980 | 5'UTR_intron|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGTTGGCGCTCCCAGGCTGTATGCCGC |
| Internal bar code: | TGTCTCGATTCCTTGATTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 99.5671 |
| LEAP-Seq n confirming: | 9200 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCGTCTACGAAGCACTCT |
| Suggested primer 2: | GATCTGTGGCTCTAGGCGTC |