| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198605 |
| Chromosome: | chromosome 17 |
| Location: | 4579278 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732901 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTCAACGCCGGCTGCAGCAGCGGCAGC |
| Internal bar code: | TGTAAGATGCCAGCGTACAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 99.1607 |
| LEAP-Seq n confirming: | 41470 |
| LEAP-Seq n nonconfirming: | 351 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCAAAGAGGGTCAACG |
| Suggested primer 2: | GTGGAGCGTTGAAAGAGAGG |