Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.198615 |
Chromosome: | chromosome 15 |
Location: | 341877 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGTTCCACGCATGGGCTTCAGCCTCAC |
Internal bar code: | GGATATGCCAGACTGGGCCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 975 |
LEAP-Seq percent confirming: | 99.655 |
LEAP-Seq n confirming: | 7799 |
LEAP-Seq n nonconfirming: | 27 |
LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGCCGTAGATGTTTGGTT |
Suggested primer 2: | TGTGTAAGCTGCGAACAAGG |