| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198616 |
| Chromosome: | chromosome 13 |
| Location: | 1272906 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570751 | CHI8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCCCTGCTCTCCTGCTGCACGGCCTGC |
| Internal bar code: | TGCCGCCCGTGGTGGTTCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 966 |
| LEAP-Seq percent confirming: | 98.6684 |
| LEAP-Seq n confirming: | 14449 |
| LEAP-Seq n nonconfirming: | 195 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACCAGACCTGACCTTGG |
| Suggested primer 2: | ATGGTAGGTTCTGGTGCAGG |