| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198635 |
| Chromosome: | chromosome 12 |
| Location: | 1589967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486551 | PFH19,PHX19 | Putative prolyl 4-hydroxylase; (1 of 1) 1.14.11.7 - Procollagen-proline 3-dioxygenase / Prolyl 3-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGCAACGCAGCGCATAGTGGTGTCAAG |
| Internal bar code: | TGTTTTCGACTTCTATTGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 813 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTCCAGGTGAGTGGTTG |
| Suggested primer 2: | AGGAGGAACATGGTGTCAGG |