| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.198652 |
| Chromosome: | chromosome 7 |
| Location: | 705667 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAGAAGGGGGAGCAAGGGGCACTTATAA |
| Internal bar code: | CGCACTAACGGGTATATCTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 99.5087 |
| LEAP-Seq n confirming: | 3038 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGTCTGCTCCCAGCTAC |
| Suggested primer 2: | CACTTGCAGTTATCGAGGCA |