Insertion junction: LMJ.RY0402.198695_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.198695
Chromosome:chromosome 7
Location:3783165
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g338350 CPLD50 Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR10072//PTHR10072:SF43 - IRON-SULFUR CLUSTER ASSEMBLY PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGACTGTCCCATCGCACATCATCTCTAT
Internal bar code:GCGCGTGGTCTGCCTCTATCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:146
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:759
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGCACAGCTGGCTGATAAAC
Suggested primer 2:TTGCATGTTGCTAGCCAGTC