| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198717 |
| Chromosome: | chromosome 6 |
| Location: | 7815700 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302650 | RRM5 | (1 of 2) 2.1.1.166 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase / Um2552 methyltransferase; Putative ribosomal RNA methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGTGCATGCAAGTCCGCGGAGGCGTGC |
| Internal bar code: | TAATTCTTCCAACGAGGAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1026 |
| LEAP-Seq percent confirming: | 99.4144 |
| LEAP-Seq n confirming: | 5772 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTCTACGCAGTGGGGTTG |
| Suggested primer 2: | TTCTTCCCCAGTCATTACGC |