Insertion junction: LMJ.RY0402.198719_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.198719
Chromosome:chromosome 16
Location:5185780
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g683100 (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGTCCAGAAGGAGTTATGGCTGCGGCGT
Internal bar code:TAGTAAACTCGCCTCGCGTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:470
LEAP-Seq percent confirming:99.7146
LEAP-Seq n confirming:2795
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCATTTGATAGACTGGGT
Suggested primer 2:GAGGTGGAGGTGGAGGTGTA