Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.198735 |
Chromosome: | chromosome_8 |
Location: | 300793 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g358564 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATTTCCCCTTCCTCCAATCGTGTGCTGCCC |
Internal bar code: | TGGCTCTTCTACCACTGTGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 235 |
LEAP-Seq percent confirming: | 91.9598 |
LEAP-Seq n confirming: | 366 |
LEAP-Seq n nonconfirming: | 32 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGGCGTTTAAGTGCCGTAT |
Suggested primer 2: | ACACAGAAGGAGCAGGAGGA |